Studies on COVID-19 cover diverse environmental segments and become complicated with time. Coronavirus disease 2019 (COVID-19) can spread through the air, and PM could act as a host to spread the virus beyond those in close contact. The spread of air pollution and viruses, two of our millennium’s most serious concerns, have been linked closely. The World Health Organization (WHO) addressed the fact that particulate pollution affects human health more severely than other air pollutants. Particulate matter (PM) is the primary health concern among these air toxins. Several new substances are being added to the air daily, polluting it and causing human health effects. Thanks in advance for any guidance, ideas, and/or discussion.Air is a diverse mixture of gaseous and suspended solid particles. My question is: How do I take the ‘intrinsic activity’ of the enzyme into account (if I need to at all)? Can anyone recommend an equation and appropriate software that can do this? We currently have SigmaPlot, GraphPad, and Enzfitter (for the double substrate inhibition model). We’ve run a full course of analyses, varying the NADH as well as the substrate and we're now trying to fit the data to an appropriate equation. The intrinsic activity introduces a baseline velocity and is complicating our kinetic analyses a bit. Complicating it even further is a noted double substrate inhibition pattern (apparently not uncommon with ping-pong mechanism enzymes). Of course, upon addition of substrate, the velocity of the reaction increases and NADH is consumed more rapidly. I’m working with a rather complicated ping pong, bi-bi double displacement reductase enzyme that oxidizes NADH and then reduces a substrate. Interestingly, the enzyme has intrinsic NADH oxidase activity in the absence of the substrate.
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